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Slideshow

Bleaching Laser

laser_gate_col1_short-new.gifTIRF microscopy of fluorescent beads. The laser spot with a diameter of ~5 µm is displayed in red.

bleach_short.GIFTIRF microscopy of a cell expressing sfGFP-tagged alpha-tubulin. A portion of the flagellum is bleach using a focused laser beam.

 

We are grateful to Drs. Ahmet Yildiz (Berkeley) and Peter Kner (UGA) for advice on how to install the bleaching laser.