2024 Dai, J., Ma, M, Niu, Q, Eisert, R.J., Wang, X., Das, P., Lechtreck, K.F., Dutcher, S.K., Zhang, R., Brown, A. (2024). Mastigoneme structure reveals insights into the O-linked glycosylation code of native hydroxyproline-rich helices. Cell March 28. doi: 10.1016/j.cell.2024.03.005. Patel, M.B., Griffin, P.J., Olson, S.F., Dai, J., Hou, Y., Malik, T, Das, P., Zhang, G., Zhao, W, Witman, G.B. and Lechtreck, K.-F. (2024). Distribution and bulk flow analyses of the IFT motor kinesin-2 support an 'on-demand' model for Chlamydomonas ciliary length control. Cytoskeleton March 8. doi: 10.1002/cm.21851. Hwang, J., Yanagisawa, H., Davis, K.C., Hunter, E.L., Fox, L.A., Jimenez, A.R., Goodwin, R. E., Gordon, S.A., Stuart, C.D.E., Bower, R., Porter, M.E., Dutcher, S.K., Sale, W.S., Lechtreck, K.F. and Alford, L.M. (2024). Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia. Cytoskeleton Jan 15 doi: 10.1002/cm.21818. Das, P, Mekonnen, B, Alkhofash, R., Ingle, A.V., Workman, E.B., Feather, A., Zhang, G., Liu, P. and Lechtreck, K.F. (2024) Small Interactor of PKD2 (SIP), a novel single-pass transmembrane protein, is required for proteolytic processing and ciliary import of Chlamydomonas PKD2. J. Cell Science 137(1):jcs261497. doi: 10.1242/jcs.261497. Lee, C., Maier, W., Jiang,Y.-Y., Nakano, K., Lechtreck, K.F. and Gaertig, J.F. (2024) Global and local functions of a Fused/Stk36 kinase, CdaH, in intracellular patterning in Tetrahymena. J. Cell Science jcs261256. doi: 10.1242/jcs.261256. 2023 Legal, T., Parra, M., Tong, M., Black, C., Joachimiak, E., Valente-Paterno, M., Lechtreck, K.F., Jacek Gaertig, and Khanh Huy Bui (2023). Molecular architecture of the ciliary tip revealed by cryo-electron tomography. J. Cell Biol. Nov 6: e202301129. doi: 10.1083/jcb.202301129. Lorentzen, E. and Lechtreck, K.-F. (2021). Chapter 13: Intraflagellar Transport. The Chlamydomonas Source Book; Volume 3. Dutcher, S.K. (Ed.) Elsevier. Mills, M.K., McCabe, L.G., Rodrigue, E.M., Lechtreck, K.F. and Starai, V.J. (2023) Wbm0076, a candidate effector protein of the Wolbachia endosymbiont of Brugia malayi, disrupts eukaryotic actin dynamics. PLOS Pathogens 19:e1010777. Saravanan, S., Trischler, Raqual Bower, Mary Porter#, and Karl Lechtreck# (2023). In vivo imaging reveals independent intraflagellar transport of the nexin-dynein regulatory complex subunits DRC2 and DRC4. Mol. Biol. Cell 34. #Co-corresponding authors. 2022 Lechtreck, K. (2022) Cargo adapters expand the transport range of intraflagellar transport. Invited review. Journal of Cell Science. 135:jcs260408. Don't have access? Get a free copy. Jin, D., Zhang, G., Alkhofash, R.A.4, Mekonnen, B.4, Saravanan, S.4, Xue, B., Fan, C.-H., Betleja, E, Cole, D.G., Liu, P., and Lechtreck, K. (2022). Loss of ARL13 impedes BBSome-dependent cargo export from Chlamydomonas cilia. Journal of Cell Biology: 221:e202201050. Lechtreck, K, Liu, Y., Dai, J., Alkhofash, R.A., Butler, J., Alford, L., and Yang, P. (2021). Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes. eLife. https://www.biorxiv.org/content/10.1101/2021.10.31.466660v1. 2021 Wingfield, J.L., Mekonnen, B., Mengoni, I., Liu, P., Jordan, M., Diener, D., Pigino, G. and Lechtreck, K. (2021). In vivo imaging shows continued association of several IFT A, B and dynein complexes while IFT trains U-turn at the tip. Journal of Cell Science 134 (18): jcs259010. Liu, Y.-X., Xue, B., Sun, W.-Y., Wingfield, J.L., Dong, B., Sun, J., Lechtreck, K.F. and Fan Z.-C. (2021). Bardet-Biedl Syndrome 3 Protein Mediates Phototaxis through Promoting Ciliary Exit of Phospholipase D via the BBSome. Elife. 2021 Feb 15;10:e59119. doi: 10.7554/eLife.59119. PMID:33587040. Zhu, X., Wang, J., Li, S., Lechtreck, K. and Pan, J. (2020) IFT54 directly interacts with kinesin-II and IFT dynein to regulate anterograde IFT. EMBO J., doi.org/10.15252/embj.2020105781 2020 Yu, K., Liu, P., Venkatachalam, D. Hopkinson, B.M. Lechtreck, K.-F. (2020) The BBSome restricts entry of tagged carbonic anhydrase 6 into the cis-flagellum of Chlamydomonas reinhardtii. PlosOne October 29. Craft Van De Weghe. J.C., Harris, J.A., Kubo, T., Witman, G.B. and Lechtreck, K.-F. (2020) Diffusion rather than IFT likely provides most of the tubulin required for axonemal assembly. J. Cell Sci. 133: jcs249805 doi: 10.1242/jcs.249805. Liu, P., Lou, X., Wingfield, J.L., Lin, J., Nicastro, D. and Lechtreck, K.-F. (2020) Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia. J Cell Biol. doi: 10.1083/jcb.202001122. Xue, B., Liu Y-X., Dong B., Wingfield, J.L., Wu, M., Sun, J., Lechtreck, K.F. and Fan Z.-C. (2020). Intraflagellar Transport Protein RABL5/IFT22 Recruits the BBSome to the Basal Body through the GTPase ARL6/BBS3. PNAS 117:2496-2505. 2019 Lechtreck, K. (2019) Dynein in Intraflagellar Transport. In: Handbook of Dynein (Second Edition). Editor: Keiko Hirose, ISBN 9789814800013. pp 251-275. Jiang, Y-Y., Maier, W., Baumeister, R., Minevich, G., Wloga, D., Ruan, Z., Kannan, N., Bocarro, S., Bahraini, A., Vasudevan, K.K., Lechtreck, K., Orias, E., and Gaertig, J. (2019) A CDK-related kinase activates LF4/MOK to regulate cilium length in Tetrahymena. PLoS Genetics 15:e1008099. Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB.(2019) A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J. Cell Sci. 132. doi: 10.1242/jcs.220749. PMID:30659111 2018 Dai, J., Barbieri, F., Mitchell, D.R., and Lechtreck, K.-F. (2018). In vivo analysis of outer arm dynein transport reveals cargo-specific IFT properties. Mol. Biol. Cell 22 Aug 2018. E18-05-0291. Wingfield, J.L. and Lechtreck, K.-F. (2018) Chlamydomonas basal bodies as flagella organizing centers. Invited review to Special Issue "Comparative Biology of Centrosomal Structures in Eukaryotes". Cells 7(7), 79; doi: 10.3390/cells7070079. Louka, P., Vasudevan, K.K., Guha, M., Joachimiak, E., Wloga, D., Tomasi, R., Baroud, C., Dupuis-Williams, P., Galati, D., Pearson,C., Rice, L., Moresco, J., Yates III, J., Jiang, Y.-Y., Lechtreck, K., Dentler, W. and Gaertig, J. (2018) Proteins that control the geometry of ciliary ends. J. Cell Biol. Sep 14. DOI: 10.1083/jcb.201804141. Lechtreck, K.-F., Mengoni, I., Okivie, B., and Hilderhoff, K.B. (2018). In vivo analyses of radial spoke transport, assembly, repair and maintenance. Cytoskeleton (in press). Hunter, E.L., Lechtreck, K., Hwang, J., Fu, G., Alford L.M., Gokhale, A., Yamamoto, R, Kamiya, R., Lin, H., Yang, F., Nicastro, D., Dutcher, S.K., Wirschell, M., and Sale, W.S. (2018). The IDA3 adapter, required for IFT transport of I1 dynein, is regulated by ciliary length. MBoC 29:886-896. Liu P. and Lechtreck, K.-F. (2018). The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export. PNAS 01.15.2017. doi: 10.1073/pnas.1713226115 2017 Liu, Y., Visetsouk, M., Mynlieff, M.,Qin, H., Lechtreck, K.-F., and Yang, P. (2017). H+- and Na+- elicited swift distinct changes of the microtubule system in heterotrophic Chlamydomonas. Elife Sep 6; doi: 10.7554/eLife.26002. Snouffer, A., Brown, D., Le, H., Walsh, J., Lupu, F., Norman, R., Lechtreck, K.-F., Ko, H.W., and Eggenschwiler, J. (2017). Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice. PLOS Genetics 13:e1006912 Wingfield, J.L., Mengoni, I., Bomberger, H., Jiang, Y.-Y., Walsh, J.D., Brown, J.M., Picariello, T., Cochran, C.A., Zhu, B., Pan, J., Eggenschwiler, J., Gaertig, J., Witman, G.B., Kner, P., and Lechtreck, K.-F. (2017). IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife May 31. doi: 10.7554/eLife.26609. Lechtreck K.-.F, Van De Weghe J.C., Harris J.A., Liu P. (2017). Protein transport in growing and steady-state cilia. Traffic 18: 277-286. 2016 Lechtreck, K.-F. (2016). Methods for Studying Movement of Molecules Within Cilia. Methods of Molecular Biology: Cilia. Eds.: S.T. Christensen & P. Satir. Volume 1454:83-96 Harris, J.A., Liu, Y., Yang, P., Kner, P., and Lechtreck, K.-F. (2016). Single particle imaging reveals IFT-independent transport and accumulation of EB1 in Chlamydomonas flagella. Molecular Biology of the Cell 27: 295-307. Kubo T, Brown JM, Bellve K, Craige B, Craft JM, Fogarty K, Lechtreck KF, Witman GB. (2016). Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. Journal of Cell Science 129:2106-19. Tran, P.V. and Lechtreck, K.-F. (2016). An age of enlightenment for cilia: The FASEB Summer Research Conference on the “Biology of Cilia and Flagella”. Meeting Report. Developmental Biology 409:319-28 2015 Lechtreck, K.-F. (2015) IFT-cargo interactions and protein transport in cilia. Trends in Biochemical Science 40:765-778. Jiang, Y.-Y., Lechtreck, K.-F. and Gaertig, J. (2015). Total Internal Reflection Fluorescence Microscopy of Intraflagellar Transport in Tetrahymena thermophila. In: Methods in Cilia & Flagella, W.F. Marshall (Ed.) Elsevier Science and Technology. Vol 127; p. 223-237. Vasudevan, K.K., Jiang Y.-Y., Lechtreck, K.-F., Kushida, Y., Alford, L.M., Sale, W.S., Hennessey, T. and Gaertig, J. (2015). Kinesin-13 regulates the quantity and quality of tubulin inside cilia. Mol. Biol Cell 26:478-94 Craft, J.M., Harris, J.A., Hyman, S., Kner, P., and Lechtreck, K.-F. (2015). Tubulin Transport by IFT is Upregulated during Ciliary Growth by a Cilium-autonomous Mechanism. J. Cell Biol. 208:223-237. (see also JCB biosights video) 2014 Awata, J.,Takada, S., Standley, C., Lechtreck, K.-F., Bellvé, K.D., Pazour, G.J., Fogarty, K.E., and Witman, G.B. (2014). Nephrocystin-4 controls ciliary trafficking of membrane and large soluble proteins at the transition zone. J. Cell Sci. 127:4714–4727. Bhogaraju, S., Weber, K., Engel, B.D., Lechtreck, K.-F. and Lorentzen, E. (2014) Getting tubulin to the tip of the cilium: One IFT train, many different tubulin cargo-binding sites? Bioassays. Lechtreck, K.F. (2014) Chlamydomonas reinhardtii as a model for flagellar assembly. Perspectives in Phycology 1: 41 - 51 (Invited review for inaugural issue). (Request reprint) 2013 Wren, K., Craft, J.M., Tritschler, D., Schauer, A., Patel,, D.K., Smith E.F., Porter, M.E., Kner, P., and Lechtreck, K.-F. (2013). A differential cargo loading model of ciliary length regulation by IFT. Current Biology 23, 2463–2471. Lechtreck, K.-F., Gould, T.J., and Witman, G.B. (2013). Repair of the Flagellar Central Pair in Chlamydomonas reinhardtii. Cilia 2,15. Lechtreck, K.-F., Brown, J.M., Sampaio, J.L., Shevchenko, A., Evans, J.E. and Witman, G.B. (2013). Cycling of the signaling protein Phospholipase D through cilia requires the BBSome only for the export phase. Journal of Cell Biology 201, 249-61. Ludington, W.B., Wemmer, K.A., Lechtreck, K.-F., Witman, G.B., and Marshall, W.F. (2013). Avalanche-like behavior in ciliary import. PNAS 110, 3925-3930. Lechtreck, K.-F. (2013). Visualizing IFT in Chlamydomonas flagella. Methods in Enzymology, 524, 265-284. pre UGA Craige, B., Tsao, C.-C., Diener, D.R., Hou, Y., Lechtreck, K.-F., Rosenbaum J.L., and Witman, G.B. (2010). CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content. Journal of Cell Biology 190, 927-940. Lechtreck, K.-F., Johnson, E.C., Sakai, T., Cochran, D., Ballif, B.A., Rush, J., Pazour, G.J., Ikebe, M., Witman, G.B. (2009). The Chlamydomonas BBSome is an IFT cargo required for export of specific signaling proteins from flagella. Journal of Cell Biology 187, 1117-1132. Lechtreck , K.-F., Luro, S., and Witman, G.B. (2009). HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex B. Cell Motil Cytoskel. 66, 469-82. Lechtreck, K.-F., Delmotte, P., Robinson, M.L., Sanderson, M.J., and Witman, G.B. (2008). Mutations in Hydin impair ciliary motility in mice. Journal of Cell Biology 180, 633-643. Lechtreck, K.-F. and Witman, G.B. (2007). Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility. Journal of Cell Biology 176, 473-482. Search articles in PubMed
